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Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis

Received: 13 October 2014     Accepted: 20 November 2014     Published: 26 November 2014
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Abstract

It has been explored the possibility of correction of prooxidant-antioxidant parameters at rats with experimental liver fibrosis and immunological status of these animals using xenogeneic embryonic stem cells. It is shown that the threefold sequential administration of copper sulfate in dose 1 mg / 100 g of body weight, which amounted to 30% of the lethal dose in 48 hour intervals between administrations induced liver fibrosis in 24 hours after the last administration to 3 month animals. It was found that the development of fibrosis, was accompanied 50 per cent increase in TBA-active products in the mitochondria and 25% of the increase in the microsomes of liver cells. Content of Ig A, IgM, circulating immune complexes (CIC) and the average molecular weight peptides was increased to 2-3 times in the same time. Introduction of xenogenic embryonic stem cells in animals with experimental fibrosis accompanied by decrease in the amount of TBA-active products to almost intact control. It coincided with the 60% increase in the activity of glutathione peroxidase in the mitochondria. Introduction of stem cells to experimental animals provide a normalization of the content of Ig A, Ig M, the CiC and the average molecular weight of the peptides.

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 6-1)

This article belongs to the Special Issue Mechanisms of Protection Against Oxidative Stress

DOI 10.11648/j.ajbls.s.2014020601.12
Page(s) 5-12
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Embryonic Stem Cells, Fibrosis, Prooxidant-Antioxidant System, Immune Parameters

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Cite This Article
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    Anatoliy Ivanovich Bozhkov, Elena Mikhajlovna Klimova, Yuriy Viktorovich Nikitchenko, Vadim Vyacheslavovich Davydov, Oxana Vladimirovna Zvyagintseva, et al. (2014). Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis. American Journal of Biomedical and Life Sciences, 2(6-1), 5-12. https://doi.org/10.11648/j.ajbls.s.2014020601.12

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    ACS Style

    Anatoliy Ivanovich Bozhkov; Elena Mikhajlovna Klimova; Yuriy Viktorovich Nikitchenko; Vadim Vyacheslavovich Davydov; Oxana Vladimirovna Zvyagintseva, et al. Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis. Am. J. Biomed. Life Sci. 2014, 2(6-1), 5-12. doi: 10.11648/j.ajbls.s.2014020601.12

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    AMA Style

    Anatoliy Ivanovich Bozhkov, Elena Mikhajlovna Klimova, Yuriy Viktorovich Nikitchenko, Vadim Vyacheslavovich Davydov, Oxana Vladimirovna Zvyagintseva, et al. Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis. Am J Biomed Life Sci. 2014;2(6-1):5-12. doi: 10.11648/j.ajbls.s.2014020601.12

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  • @article{10.11648/j.ajbls.s.2014020601.12,
      author = {Anatoliy Ivanovich Bozhkov and Elena Mikhajlovna Klimova and Yuriy Viktorovich Nikitchenko and Vadim Vyacheslavovich Davydov and Oxana Vladimirovna Zvyagintseva and Natalia Igorevna Kurguzova and Vadim Ivanovich Sidorov and Alexandr Vladimirovich Naglov},
      title = {Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {2},
      number = {6-1},
      pages = {5-12},
      doi = {10.11648/j.ajbls.s.2014020601.12},
      url = {https://doi.org/10.11648/j.ajbls.s.2014020601.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.s.2014020601.12},
      abstract = {It has been explored the possibility of correction of prooxidant-antioxidant parameters at rats with experimental liver fibrosis and immunological status of these animals using xenogeneic embryonic stem cells. It is shown that the threefold sequential administration of copper sulfate in dose 1 mg / 100 g of body weight, which amounted to 30% of the lethal dose in 48 hour intervals between administrations induced liver fibrosis in 24 hours after the last administration to 3 month animals. It was found that the development of fibrosis, was accompanied 50 per cent increase in TBA-active products in the mitochondria and 25% of the increase in the microsomes of liver cells. Content of Ig A, IgM, circulating immune complexes (CIC) and the average molecular weight peptides was increased to 2-3 times in the same time. Introduction of xenogenic embryonic stem cells in animals with experimental fibrosis accompanied by decrease in the amount of TBA-active products to almost intact control. It coincided with the 60% increase in the activity of glutathione peroxidase in the mitochondria. Introduction of stem cells to experimental animals provide a normalization of the content of Ig A, Ig M, the CiC and the average molecular weight of the peptides.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis
    AU  - Anatoliy Ivanovich Bozhkov
    AU  - Elena Mikhajlovna Klimova
    AU  - Yuriy Viktorovich Nikitchenko
    AU  - Vadim Vyacheslavovich Davydov
    AU  - Oxana Vladimirovna Zvyagintseva
    AU  - Natalia Igorevna Kurguzova
    AU  - Vadim Ivanovich Sidorov
    AU  - Alexandr Vladimirovich Naglov
    Y1  - 2014/11/26
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbls.s.2014020601.12
    DO  - 10.11648/j.ajbls.s.2014020601.12
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 5
    EP  - 12
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.s.2014020601.12
    AB  - It has been explored the possibility of correction of prooxidant-antioxidant parameters at rats with experimental liver fibrosis and immunological status of these animals using xenogeneic embryonic stem cells. It is shown that the threefold sequential administration of copper sulfate in dose 1 mg / 100 g of body weight, which amounted to 30% of the lethal dose in 48 hour intervals between administrations induced liver fibrosis in 24 hours after the last administration to 3 month animals. It was found that the development of fibrosis, was accompanied 50 per cent increase in TBA-active products in the mitochondria and 25% of the increase in the microsomes of liver cells. Content of Ig A, IgM, circulating immune complexes (CIC) and the average molecular weight peptides was increased to 2-3 times in the same time. Introduction of xenogenic embryonic stem cells in animals with experimental fibrosis accompanied by decrease in the amount of TBA-active products to almost intact control. It coincided with the 60% increase in the activity of glutathione peroxidase in the mitochondria. Introduction of stem cells to experimental animals provide a normalization of the content of Ig A, Ig M, the CiC and the average molecular weight of the peptides.
    VL  - 2
    IS  - 6-1
    ER  - 

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Author Information
  • Biology Research Institute of V. N. Karazin Kharkov National University, Kharkov, Ukraine

  • State Institution “Institute of General and Urgent Surgery NAMS of Ukraine”, Kharkov, Ukraine

  • Biology Research Institute of V. N. Karazin Kharkov National University, Kharkov, Ukraine

  • State Institution “Institute of Children and Adolescent Health NAMS of Ukraine”, Kharkov, Ukraine

  • State Institution “Institute of General and Urgent Surgery NAMS of Ukraine”, Kharkov, Ukraine

  • Biology Research Institute of V. N. Karazin Kharkov National University, Kharkov, Ukraine

  • Biology Research Institute of V. N. Karazin Kharkov National University, Kharkov, Ukraine

  • Biology Research Institute of V. N. Karazin Kharkov National University, Kharkov, Ukraine

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