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Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L)

Received: 9 March 2015     Accepted: 22 March 2015     Published: 26 March 2015
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Abstract

The diamine oxidase enzyme (DAO) can be isolated from mung bean sprouts (Vigna radiata L) through the process of extraction in phosphate buffer. Concentration and fractionation were performed by the addition of ammonium sulphate, (NH4)2SO4 saturation in stages and cold centrifuged at 10000 rpm. Enzyme activity of DAO crude and DAO fractionation results were tested using substrat histamin and measured with a spectrophotometer at a wavelength of 450 nm. This study revealed that the enzyme activity of the crude enzyme was1, 226 mU/mL, the fraction of 40-60% ammonium sulphate saturated had the highest activity which accounted for 3326 mU/mL and after gel filtration, the results for both activity and specific activity were 116 mU/mL and 185 mU/mg protein.

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 1)
DOI 10.11648/j.ajbls.20150301.12
Page(s) 7-11
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Diamine Oxidase, Mung Bean, Fractionation, Ammonium Sulphate

References
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[2] Choudhary, A., Singh, I. and Sigh, R. P., 1999, A thermostable Diamine Oxidase from Vignaradiata Seedlings. Phytochemistry. 52: 1-5.
[3] Choudhary, A., Singh, R.P., 2013, Cadmium-induced Changes in Diamine Oxidase Activity and Polyamine Levels in VignaradiataWilczek Seedlings, Journal of Plant Physiology, 156: 704-710
[4] Gaman, P.M and K.B. Sherrington.(1994). Ilmu Pangan, Pengantar Ilmu Pangan, Nutrisidan Mikrobiologi. Universitas Gadjah Mada press. Yogyakarta.
[5] Lee, J. M. (1992). Biochemical Engineering.Prentice Hall Inc. New Jersey.
[6] Medda, R., Padiglia, A. and Floris, G.,1995, Plant Copper amine Oxidases. Phytochemistry. 39: 1-9.
[7] Mulchandani, A. and Rogers, R., 2001, Enzyme and Microbial Biosensors; Techniques and Protocols, Humana Press Inc., New Jersey
[8] Mutiah, D., 2005, Ultrafiltrasi, Presipitasi Bertingkat dan Kromatografi Penukar Ion sebagai Tahapan Pemurnian Enzim Protease Bacillus megaterium MS-961, Fakultas Teknologi Pertanian Institut Pertanian Bogor.
[9] Murray, R.K., Granner, D.K., and Victor, R.W., 2009, Biokimia Harper, EGC Penerbit Buku Kedokteran, Jakarta, 68-69
[10] Padiglia, A., Cogoni, A.and Floris, G., 1991, Characterization of Amine Oxidases from Pisum, Lens, Lathyrus and Cicer. Phytochemistry. 30: 3895-3897
[11] RINALDl, A., FLORIS, G.,and FINAZZI, A., 1982, Purification and Properties of Diamine Oxidase from Euphorbia latex, Eur. J. biochem, 127, 417-422
[12] Suresh, M. R., Ramakrishna, S. and Adiga, P. R., 1976, Diamine Oxidase of Lathyrussativus Seddlings. Phytochemistry. 15: 483-485.
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[15] Wimmerova. M. and Macholan, L., 1999, Sensitive Amperometric Biosensor for the Determination of Biogenic and Synthetic Amines Using Pea Seedlings Amine Oxidase: A Novel Approach for Enzyme Immobilization. Biosensor and Bioelectronics. 14: 695-702
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    Abdul Karim, Abd Rauf Patong, Abd Wahid Wahab, Indah Raya. (2015). Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L). American Journal of Biomedical and Life Sciences, 3(1), 7-11. https://doi.org/10.11648/j.ajbls.20150301.12

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    ACS Style

    Abdul Karim; Abd Rauf Patong; Abd Wahid Wahab; Indah Raya. Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L). Am. J. Biomed. Life Sci. 2015, 3(1), 7-11. doi: 10.11648/j.ajbls.20150301.12

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    AMA Style

    Abdul Karim, Abd Rauf Patong, Abd Wahid Wahab, Indah Raya. Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L). Am J Biomed Life Sci. 2015;3(1):7-11. doi: 10.11648/j.ajbls.20150301.12

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  • @article{10.11648/j.ajbls.20150301.12,
      author = {Abdul Karim and Abd Rauf Patong and Abd Wahid Wahab and Indah Raya},
      title = {Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L)},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {3},
      number = {1},
      pages = {7-11},
      doi = {10.11648/j.ajbls.20150301.12},
      url = {https://doi.org/10.11648/j.ajbls.20150301.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150301.12},
      abstract = {The diamine oxidase enzyme (DAO) can be isolated from mung bean sprouts (Vigna radiata L) through the process of extraction in phosphate buffer. Concentration and fractionation were performed by the addition of ammonium sulphate, (NH4)2SO4 saturation in stages and cold centrifuged at 10000 rpm. Enzyme activity of DAO crude and DAO fractionation results were tested using substrat histamin and measured with a spectrophotometer at a wavelength of 450 nm. This study revealed that the enzyme activity of the crude enzyme was1, 226 mU/mL, the fraction of 40-60% ammonium sulphate saturated had the highest activity which accounted for 3326 mU/mL and after gel filtration, the results for both activity and specific activity were 116 mU/mL and 185 mU/mg protein.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L)
    AU  - Abdul Karim
    AU  - Abd Rauf Patong
    AU  - Abd Wahid Wahab
    AU  - Indah Raya
    Y1  - 2015/03/26
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajbls.20150301.12
    DO  - 10.11648/j.ajbls.20150301.12
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 7
    EP  - 11
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20150301.12
    AB  - The diamine oxidase enzyme (DAO) can be isolated from mung bean sprouts (Vigna radiata L) through the process of extraction in phosphate buffer. Concentration and fractionation were performed by the addition of ammonium sulphate, (NH4)2SO4 saturation in stages and cold centrifuged at 10000 rpm. Enzyme activity of DAO crude and DAO fractionation results were tested using substrat histamin and measured with a spectrophotometer at a wavelength of 450 nm. This study revealed that the enzyme activity of the crude enzyme was1, 226 mU/mL, the fraction of 40-60% ammonium sulphate saturated had the highest activity which accounted for 3326 mU/mL and after gel filtration, the results for both activity and specific activity were 116 mU/mL and 185 mU/mg protein.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Laboratory of Biochemistry, Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Makassar, Indonesia

  • Laboratory of Biochemistry, Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Makassar, Indonesia

  • Laboratory of Analytic chemistry, Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Makassar, Indonesia

  • Laboratory of Inorganic chemistry, Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Makassar, Indonesia

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